What this means
5324, including four previously unreported compounds. All isolates were structurally elucidated using NMR spectroscopy and HR-ESI-MS analyses.
A plain-English summary is on its way. Until then, this is the start of the authors' own summary.
- Who it was tested in
- Not stated
- Kind of research
- A study
The science
- Original title
- 11α-hydroxylated derivatives of lithocholic acid produced by microbial biotransformation and their anti-aging effects.
- Authors
- Wang W, Shi Y, Liu Y et al.
- Journal
- Steroids
- Published
- Oct 22, 2026
- Study type
- Study
- PMID
- 42906741 ↗
Abstract
Seven 11α-hydroxylated lithocholic acid (LCA) derivatives were produced via catalytic biotransformation by Aspergillus ochraceus CGMCC 3.5324, including four previously unreported compounds. All isolates were structurally elucidated using NMR spectroscopy and HR-ESI-MS analyses. A. ochraceus efficiently catalyzed multiple structural modifications on the LCA skeleton, including hydroxylation, methyl esterification, and acetylation, thereby expanding the structural diversity of LCA derivatives. Furthermore, the anti-aging activities of these biotransformed metabolites were evaluated in a Caenorhabditis elegans model by measuring lifespan and locomotor behavior. Most transformed derivatives exhibited superior anti-aging potency relative to the parent LCA. Methyl and acetyl modifications markedly improved nematode lifespan and locomotor performance, while C-12β hydroxylation reduced the bioactivity of LCA derivatives. These findings demonstrate that microbial biotransformation is a robust strategy for the structural diversification and bioactivity optimization of LCA.
Wang W, Shi Y, Liu Y et al. 11α-hydroxylated derivatives of lithocholic acid produced by microbial biotransformation and their anti-aging effects. Steroids. 2026. doi:10.1016/j.steroids.2026.109857. PMID: 42906741. https://pubmed.ncbi.nlm.nih.gov/42906741/